Pracownie badawcze

Prof. dr hab. Jacek Hennig

Pracownia Patogenzy Roślin

Zakres badań

The long-term goal of our studies is to unravel disease-resistance signaling pathways in the Solanaceae family. Interactions between potato and its pathogen potato virus Y (PVY) are the principal, although not exclusive, model systems used.

Badania

Najważniejsze osiągnięcia badawcze

  • We found that the TNL immune receptor Rysto recognizes the PVY coat protein and is required for PVY-triggered immunity. The expression of Rysto confers an extreme resistance response in potato that stops viral replication without triggering cell death.
  • We used high-throughput phenotyping and gene expression analyses to search for relationships between morpho-physiological and molecular responses to water deficits.

Opis badań

In response to pathogen infection, plant cells initiate a signaling cascade that leads to changes in expression profiles of many genes and consequently the reprogramming of cellular metabolism. To establish a successful defense strategy, this response must occur quickly and effectively. Therefore, the recognition of pathogen-derived signals appears to be a critical process.

The aim of our laboratory is to reveal critical components of plant defense signaling networks using cutting-edge approaches in molecular biology and biochemistry. The availability of potato genotypes that differ in their response to PVY is a good basis to identify their resistance sources. Classic approaches, even assisted by data that were acquired from the Potato Genome Sequencing Consortium, did not result in expanding a list of agriculturally suitable DNA sequences.

A useful technique that combines target enrichment (TE) and next-generation sequencing (NGS) was developed to accelerate the cloning of genes from large eukaryotic genomes. We applied this technique to isolate and characterize the Rysto gene that is responsible for the recognition of PVY upon infection that in turn initiates the activation of a pathway that leads to extreme resistance. In parallel to this high-throughput molecular approach, we identified PVY coat protein (CP) as an avirulence factor in Rysto-triggered immunity. To precisely map a minimal CP fragment that is sensed by Rysto,, we created a series of constructs that encode truncated CP variants. We found that any deletion that affected the CP core abolished virus perception, indicating that an intact CP core was required for the Rysto-dependent resistant response. In the future, we would like to concentrate on elucidating the mechanisms of CP recognition by Rysto. We will also focus on the nature of effectors that are involved in direct or indirect Rysto-CP interactions.

Additionally, we developed a biological system to provide new insights into the extreme resistance type of defense. It is extremely important to assess whether the introduction of Rysto as a single element into other potato varieties or into different plant species (e.g., tomato, tobacco, eggplant) activates entire signaling cascades that lead to the establishment of extreme resistance.

In parallel to the main scientific interests of our laboratory, we also developed two small projects. In the first project, we applied high-throughput phenotyping and gene expression analyses to search for relationships between morpho-physiological and molecular responses of plants to water deficits. The second project focused on the characterization of a novel calcium binding protein that is involved in pollen development, with a special emphasis on its role in ribosomal RNA metabolism.

Metodologia

Our methods and methodology include various plant models that belong to the Solanaceae family (Solanum tuberosum, Solanum americanum, Nicotiana tabacum, and Nicotiana benthamiana), different systems for gene isolation and characterization and its expression in prokaryotic or eukaryotic cells, high-throughput phenotyping of plants, resistance gene enrichment sequencing (RenSeq) and PacBio SMRT (Pacific Biosciences Single-Molecule Real Time Sequencing), live-Cell imaging of green fluorescent protein (GFP) in plants, the use of GFP to study viral infections, CRISPR/Cas9-mediated targeted mutagenesis of potato, and a live-Tissue quantitative assay for assessing the hypersensitive response (resistance).

Wybrane publikacje

  • Baebler Š., Witek K., Petek, K. Stare K., Tušek-Žnidarič M. Pompe-Novak M., Renaut J., Szajko K., Strzelczyk-Żyta D., Marczewski W., Morgiewicz K.,  Gruden K. and Hennig J*. (2013) Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato. J. Exp. Bot. (2014) 65(4): 1095-1109 doi: 10.1093/jxb/ert447.
  • Rymaszewski W., Vile D., Bediee A., Dauzat M., Luchaire N., Kamrowska D., Granier Ch. and Hennig J*. (2017) Stress-Related Gene Expression Reflects Morphophysiological Responses to Water Deficit. Plant Physiol. 174 (3): 1913-1930 doi: 10.1104/pp.17.00318.
  • Grech-Baran M., Witek K., Szajko K., Witek A., Morgiewicz K., Wasilewicz-Flis I., Jakuczun H., Marczewski W., Jones J.D.G. and Hennig J*. (2020) Extreme resistance to Potato Virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor. Plant Biotech. J. 18(3): 655-667 doi: 1111/pbi.13230.

Współpraca

  • Jonathan D.G. Jones, The Sainsbury Laboratory, University of East Anglia, Norwich, UK. (tsl.ac.uk/staff/jonathan-jones/)
  • Kristina Gruden, National Institute of Biology, Dept. for Biotechnology and Systems Biology, Ljubljana, Slovenia
  • Waldemar Marczewski, The Plant Breeding and Acclimatization Institute (IHAR) – National Research Institute, Młochów, Poland
  • Christine Granier, Laboratoire d’Ecophysiologie des Plantes sous Stress Environnementaux, Institut National de la Recherche Agronomique-Supagro, Montpellier, France

Nagrody i wyróżnienia

  • Wojciech Rymaszewski, Short Term Fellowship. 2013. EMBO, Germany.
  • Marta Grech-Baran. AgroBioTop Prize. 2019. The Committee of Biotechnology of the Polish Academy of Sciences, Poland.

Publikacje

HOSER R., LICHOCKA M., ŻURCZAK M., HENNIG J., KRZYMOWSKA M., Emerging role of SGT1 as a regulator of NB-LRR-receptor nucleocytoplasmic partitioning. Plant Signaling & Behavior (2014) 9(4): e28724 (4p.) http://dx.doi.org/10.4161/psb.28724 IF -
BAEBLER Š., WITEK K., PETEK M., STARE K., TUŠEK-ŽNIDARIČ M., POMPE-NOVAK M., RENAUT J., SZAJKO K., STRZELCZYK-ŻYTA D., MARCZEWSKI W., MORGIEWICZ K., GRUDEN K., HENNIG J., Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato. Journal of Experimental Botany (2014) 65(4): 1095-1109 IF 5.794
HOSER R., ŻURCZAK M., LICHOCKA M., ZUZGA S., DADLEZ M., SAMUEL M.A., ELLIS B.E., STUTTMANN J., PARKER J.E., HENNIG J., KRZYMOWSKA M., Nucleocytoplasmic partitioning of tobacco N receptor is modulated by SGT1. New Phytologist (2013) 200: 158-171 IF 6.736
PIECZYŃSKI M., MARCZEWSKI W., HENNIG J., DOLATA J., BIELEWICZ D., PIONTEK P., WYRZYKOWSKA A., KRUSIEWICZ D., STRZELCZYK-ŻYTA D., KONOPKA-POSTUPOLSKA D., KRZESŁOWSKA M., JARMOŁOWSKI A., SZWEYKOWSKA-KULIŃSKA Z., Down-regulation of CBP80 gene expression as a strategy to engineer a drought-tolerant potato. Plant Biotechnology Journal (2013) 11(4): 459-469 IF 6.279
GISKA F., LICHOCKA M., PIECHOCKI M., DADLEZ M., SCHMELZER E., HENNIG J., KRZYMOWSKA M., Phosphorylation of HopQ1, a type III effector from Pseudomonas syringae, creates a binding site for host 14-3-3 proteins. Plant Physiology (2013) 161(4): 2049-2061 IF 6.555
WOJTKOWIAK A., WITEK K., HENNIG J., JASKÓLSKI M., Structures of an active-site mutant of a plant 1,3-β-glucanase in complex with oligosaccharide products of hydrolysis. Acta Crystallographica Section D Biological Crystallography (2013) 69(Pt 1): 52-62 IF 14.103
WOJTKOWIAK A., WITEK K., HENNIG J., JASKÓLSKI M., Two high-resolution structures of potato endo-1,3-β-glucanase reveal subdomain flexibility with implications for substrate binding. Acta Crystollographica Section D Biological Crystallography (2012) 68(Pt 6): 713-723 IF 12.619
MAASSEN A., HENNIG J., Effect of Medicago sativa Mhb1gene expression on defense response of Arabidopsis thaliana plants. Acta Biochimica Polonica (2011) 58(3): 427-432 IF 1.234
CLARK G., KONOPKA-POSTUPOLSKA D., HENNIG J., ROUX S., Is annexin 1 a multifunctional protein during stress responses? Plant Signaling & Behavior (2010) 5(3): 1-5 IF-

Patenty

  • Witek K., Grech-Baran M., Hennig J., Jones J.D.G., Marczewski W. and Szajko K. POTYVIRUS RESISTANCE GENES AND METHODS OF USE No: 62/538,020 to US Patent and Trademark Office (28.07.2018).