Collection of siRNA
Loss-of-function screens are valuable tools used to discover genes involved in specific biological processes or to identify processes where simultaneous inactivation increases the sensitivity of particular cell types, such as cancer cells.
One of the primary methods for inducing gene loss-of-function is through RNA interference (RNAi). In this process, short interfering RNA (siRNA) hybridizes to its complementary messenger RNA (mRNA) inside the cell, leading to the degradation of the mRNA and preventing the synthesis of the corresponding protein. This method is often employed in high-throughput procedures where chemically synthesized siRNAs are introduced into cultured mammalian cells. It is frequently combined with high-throughput fluorescence microscopy, allowing for the simultaneous monitoring of various biological processes (HCS, high-content screening).
The RNA Biology Laboratory at IBB PAS has a genome-wide siRNA library that targets nearly all protein-coding genes in humans. Additionally, based on literature and our analyses, we have developed smaller libraries containing siRNAs for genes involved in specific biological processes. Application of targeted libraries reduces screening costs, minimizes the experimental effort required, and shortens the screening time.
We posses the following siRNA libraries:
- Whole-genome library (18,106 genes)
- Mitochondrial biology
- RNA biology
- Protein homeostasis
- Trafficking
As part of scientific collaboration or as a commercial service, we offer support in planning screens, developing high-throughput readouts, and executing the screening process. Our laboratory is equipped with the necessary instruments to handle libraries and to culture and manipulate cells in 96-well or 384-well formats. We also perform readouts using high-content screening (HCS), quantitative fluorescence microscopy, or multi-well plate readers. This instrumental setup enables efficient and reliable testing of multiple culturing conditions simultaneously.
Researchers from academic institutions or R&D companies are invited to contact dr. Przemysław Surowiecki (szczesnylab@ibb.waw.pl).