Collection of Arabidopsis mutants (Koncz collection)

One of the largest mutants collections, the Koncz collection of T-DNA tagged Arabidopsis thaliana plants (Ríos et al., 2002), includes 92,500 independent transgenic lines, with an average number of 1.3 copies of T-DNA insertion per line, represented by pools of 100 plants.  This collection can be screened for single mutant lines using a simple and fast PCR-based method that identifies genes disrupted by the T-DNA insertion carrying a hygromycin-resistance marker. Using this technique, an M₂ family segregating a characterized gene mutation can be identified within 4 weeks.

The collection – which has been donated by the Max-Planck Institute for Plant Breeding Research (Cologne, Germany) – is now available for screening in the Laboratory of Gene Expression Regulation at the Institute of Biochemistry and Biophysics PAS, in a collaborative manner.

For further details and substantive assistance please contact the Head of the Laboratory, Prof. Tomasz Sarnowski, tsarn@ibb.waw.pl.

Reference:

Ríos G, Lossow A, Hertel B, Breuer F, Schaefer S, Broich M, Kleinow T, Jásik J, Winter J, Ferrando A, Farrás R, Panicot M, Henriques R, Mariaux JB, Oberschall A, Molnár G, Berendzen K, Shukla V, Lafos M, Koncz Z, Rédei GP, Schell J, Koncz C. (2002) Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes. Plant J. 32(2):243-53. doi: 10.1046/j.1365-313x.2002.01416.x